ISO INTERNATIONAL STANDARD 18363-1 First edition 2015-08-15 Animal and vegetable fats and oils - Determination of fatty-acid-bound chloropropanediols (MCPDs) and glycidol by GC/MS - Part 1: Method using fast alkaline transesterification and measurement for3-McPD anddifferential measurement for glycidol Corps gras d'origines animale etvegétale-Détermination des estersdechloropropanediols (MCPD)etd'acidesgrasetdesestersde glycidol et d'acides gras Partie1:Meéthodepartransesterificationalcalinerapideet mesure pourle chloro-3 propane-1,2-diol (3-MCPD) et par mesure differentielle pour le glycidol Reference number ISO18363-1:2015(E) TSO Intemational Organization for Standardization @IS02015 from IHS IS018363-1:2015(E) COPYRIGHTPROTECTEDDOCUMENT IS02015,Published inSwitzerland All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below or ISO's member body in the country of the requester. ISO copyright office Ch. de Blandonnet 8. CP 401 CH-1214 Vernier, Geneva, Switzerland Tel. +41 22 749 01 11 Fax +4122 749 09 47 [email protected] www.iso.org Intemationaibr PrganizationforStandardization etworking permitted without license from IHS NotforResale, 2015/11/1108:21:3 IS018363-1:2015(E) Contents Page Foreword ..iv Introduction.. 1 Scope.. 2 Normativereferences 3 Terms and definitions ..1 4 Principle ..2 5 Reagents .3 5.1 General .3 5.2 Standard and reference compounds .3 5.3 Solvents .3 5.4 Other reagents .3 6 Apparatus ..4 7 Sample. .4 7.1 Sampling. .4 7.2 Preparation ofthetest sample .4 8 Procedure 5 8.1 Spiking with surrogate standard and homogenization .5 8.2 Ester cleavage and glycidol transformation. .5 8.3 Matrix clean up .5 8.4 Derivatization .5 8.5 Gaschromatography/massspectrometryreferences 6 9 Expression of results .6 10 Precision .1 10.1 Interlaboratorytest. ..7 10.2 Repeatability .8 10.3 Reproducibility .8 11 Test report ..8 Annex A (informative) Results of an collaborative trial .9 Bibliography .11 iii nsee=Zhejiang Institute of Stan ithout license from IHS