ANSI NSF International Standard/ AmericanNationalStandard NSF/ANSI 49 - 2022 Biosafety Cabinetry: Design, Construction, Performance, and Field Certification NSF NSF International, an independent, not-for-profit, nongovernmental organization, is dedicated to beingtheleadingglobalproviderofpublichealth and safety-based risk management solutions while serving the interests of all stakeholders. This standard is subject to revision. Contact NSF to confirm this revision is current. Users of this standard may request clarifications and interpretations, or propose revisions by contacting: Chair, Joint Committee on Biosafety Cabinetry c/oNSFInternationai 789 North Dixboro Road, P.O. Box 130140 Ann Arbor, Michigan 48113-0140 U.S.A. Phone: (734) 769-8010 Fax: (734) 769-0109 Email: [email protected] Web: <www.nsf.orq> NSF/ANSI 49 - 2022 NSF International Standard / American National Standard for Biosafety Cabinery - Biosafety Cabinetry: Design, Construction, Performance, and Field Certification Standard Developer NSF International Designated as an ANSI Standard August 9, 2022 AmericanNationalStandardsInstitute - Prepared by The NSF Joint Committee on Biosafety Cabinetry Recommended for adoption by TheNSFCouncilofPublicHealthConsultants Adopted by NSF International June 1976 Revised May 1983 Revised June 1987 Revised May 1992 Revised March 2002 Addendum November 2002 Revised February 2004 Revised September 2004 AddendumOctober2004 Addendum March 2005 Revised July 2007 Revised October 2008 Revised June 2009 Revised September 2010 Revised November 2010 Revised November 2011 Revised July 2012 Revised February 2015 Revised March 2017 Revised January 2019 Revised March 2020 Revised March 2021 Revised February 2023 Published by NSF International P.O. Box 130140, Ann Arbor, Michigan 48113-0140, U.S.A. For ordering copies or for making inquiries with regard to this standard, please reference the designation "NSF/ANSI49-2022." Copyright 2022 NSF International 1992,1987,1983,1976 means, electronic or mechanical, including photocopying and microfilm, without permission in writing from NSF International. Printed in the United States of America. ii Disclaimers NSFInternational (NSF),inperforming itsfunctions in accordancewith itsobjectives,doesnotassume or of NSF represent its professional judgment. NSF shall not be responsible to anyone for the use of or reliance consequential damages, arising out of or in connection with the use, interpretation of, or reliance upon this standard. It is the responsibility of the user of this standard to judge the suitability of the ANS for the user's purpose. Participation in NsF standards development activities by regulatory agency representatives (federal, state, or local) shall not constitute their agency's endorsement of NSF or any of its standards. Preference is given to the use of performance criteria measurable by examination or testing in materials,or construction criteria. The illustrations,if provided,are intended toassist in understanding their adjacent standard requirements However, the illustrations may not include all requirements for a specific product or unit, nor do they show theonlymethod offabricatingsucharrangements.Suchpartial drawingsshall not be usedtojustify improper or incomplete design and construction. This information is given for the convenience of users of this standard and does not constitute an organization 1 The information contained in this disclaimer is not part of this American National Standard (ANS) and has not been has not been subjected to public review or a consensus process. In addition, it does not contain requirements necessary forconformancetothe standard ii Disclaimers NSFInternational (NSF),inperforming itsfunctions in accordancewith itsobjectives,doesnotassume or of NSF represent its professional judgment. NSF shall not be responsible to anyone for the use of or reliance consequential damages, arising out of or in connection with the use, interpretation of, or reliance upon t