ISO INTERNATIONAL STANDARD 9184-5 First edition 1990-12-15 Paper, board and pulps -- Fibre furnish analysis Part 5: Lofton-Merritt staining test (modification of Wisbar) Papier,cartonetpates-Determinationdelacompositionfibreuse Partie5:Colorationde Lofton-Merritt (modification de Wisbar) Referencenumber ISO9184-5:1990(E) ISO 9184-5:1990(E) Foreword iso (the International Organization for Standardization)is a woridwide federation of national standards bodies (isoO member bodies).The work of preparing International Standards is normally carried out through iso technical committees. Each member body interested in a subject for which a technical committee has been established has the right to be represented on that committee. International organizations, govern- mental and non-governmental, in liaison with isO,also takepart in the work.Iso collaborates closely with the International Electrotechnical Commission (IEC)on all matters of electrotechnical standardization. Draft International Standards adopted by the technical committees are circulated to the member bodies for voting. Publication as an Inter- national Standard requires approval by at least 75% of the member bodies casting a vote. International Standard Iso9184-5waspreparedbyTechnical Committee ISO/TC6,Paper,boardandpulps. iso 9184 consists of the foliowing parts,under the general title Paper, board andpulps -Fibre furnish analysis: Part 1:General method - -Part 2:Staining guide -Part3:Herzberg stainingtest --Part 4:Graff"C"stainingtest Part 5:Lofton-Merritt staining test (modification of Wisbar) Part6:Weightfactorsbyfibrecoarsenessmethod Part7:Weightfactorsbycomparisonmethod Part 1 gives general instructions for the performance of fibre furnish analysis. It should be used in conjunction with the staining guide (see part 2) and the staining tests (see parts 3 to 5). Additional parts of this International Standard will be published if re- quired by the development of new kinds of fibres ornew staining tests. Annex A of this part of Iso 9184 is for information only ISO1990 permission in writing from the publisher. Internationa!Organization for Standardization Case Postale56.CH-1211Geneve20·Switzerland Printed in Switzerland INTERNATIONAL STANDARD ISO 9184-5:1990(E) Paper, board and pulps --- Fibre furnish analysis Part 5: Lofton-Merritt staining test (modification of Wisbar) Scope 4 Reagents 1 Thispartoftso9184specifiesthepreparation,use CAUTION -- Some of the components used in pre- and colour reactions of Lofton-Merritt stain in fibre paring this stain are toxic. The stain should be pre- furnish analysis. It should be used in conjunction pared and handled in keeping with safe laboratory withisO 9184-1and, if necessary,with other staining practice, tests defined in Iso 9184-2. Use only reagents of recognized analytical grade The Lofton-Merritt staining test is applicable to the and distilledwater orwaterof equivalentpurity qualitative and quantitative differentiation of the fol- lowing puips: 4.1 Fuchsine,solution,about1% (m/m) unbleached and bleached softwood chemical Add 1 g of fuchsine monohydrochloride (C2oH2oNgCl) :sdind (C.1. 42510 --- C.1. basic violet 14) in small portions to 50 ml of boiling water in a 250 ml beaker, with vig- unbleachedkraft and sulfite in softwood pulps; orous stirring.Dilute to 100 ml. unbieached semi-chemical kraft and sulfite pulps. 4.2Malachitegreen,solution,about2% (m/m) Take 2 g of malachite green (C23H25N,CI) (C.I. 42000 Normativereference C.I.basicgreen4)andproceed asdescribedfor the fuchsine solution. The following standard contains provisions which through reference inthistext,constitute provisions of this part of Iso 9184.At the time of publication. 4.3 Hydrochloric acid, solution, about 0,5 % (m/m) theedition indicated was valid.All standards are Dilute 5ml of a 37% (m/m) solution of hydrochloric subjectto revision,and parties to agreements based acid (HCI) to 400 ml. on this part of iso 9184 are encouraged to investi- gate the possi