ISO INTERNATIONAL STANDARD 22283 First edition 2020-08 Traditional Chinese medicine : Determination of aflatoxins in natural products by LC-FLD Medecine traditionnelle chinoise-Dosagedes aflatoxines dans les produits naturels par CL-DF Reference number ISO 22283:2020(E) ISO @ISO 2020 IS0 22283:2020(E) COPYRIGHTPROTECTEDDOCUMENT @IS02020 All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting below or Iso's memberbody in the country ofthe requester. ISO copyright office CP 40i : Ch.de Blandonnet 8 CH-1214 Vernier, Geneva Phone: +41 22.749 01 11 Email: copyright@iso.org Website: www.iso.org Published in Switzerland ii @ IS0 2020 - All rights reserved IS022283:2020(E) Contents Page Foreword ..iv Introduction .. 1 Scope. ..1 2 Normative references 3 Terms and definition 4 Symbols and abbreviated terms ..1 5 Reagents .2 6 Apparatus .2 6.1 LC-FLD .2 Chromatographiccolumn 2 6.2 6.3 Glass sample 2 6.4 Electronic balance .2 6.5 Homogenizer .2 6.6 Centrifuge.. .2 6.7 Volumetric flask 2 7 Sample preparation. .2 8 Test method. .3 8.1 Stocksolutionand working solution .3 8.2 LC-FLD conditions. .3 8.2.1 .3 General. 8.2.2 LC-FLD conditions and system suitability .3 8.2.3 Post-columnderivatization .4 8.2.4 Quantificationofaflatoxins inthetestsampleusingcalibrationcurves. 4 8.3 Application of test method .4 9 Sampling and preservation 9.1 Sampling. .5 9.2 Sample storage ..5 AnnexA(informative)LC-FLDmethod. ..6 Annex B (informative) Chromatogram of AFG2,AFG, AFB2 and AFB, Annex C (informative) Reference ofnational, regional and organizational limits of aflatoxinsinnaturalproducts .9 AnnexD(informative)Methodvalidation .11 ..12 Bibliography @IS0 2020 -All rights reserved ili