ISO INTERNATIONAL STANDARD 20579-3 First edition 2021-04 Surface chemical analysis - Sample handling, preparation and mounting - Part 3: Biomaterials Analysechimiquedessurfaces-Manipulation,préparation et montagedeséchantillons- Partie3:Biomatériaux Reference number IS0 20579-3:2021(E) ISO @IS0 2021 IS020579-3:2021(E) COPYRIGHTPROTECTEDDOCUMENT IS02021 All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may below or ISo's member body in the country of the requester. ISO copyright office CP 40i : Ch. de Blandonnet 8 CH-1214 Vernier, Geneva Phone: +41 22 749 01 11 Email: copyright@iso.org Website: www.iso.org Published in Switzerland ii IS02021-Allrights reserved IS020579-3:2021(E) Contents Page Foreword iv Introduction. V 1 Scope. .1 2 Normativereferences .1 3 Terms and definitions .1 4 Abbreviatedterms. .2 5 Explanation of the structure of this document .2 6 General requirements and classes of specimens .3 6.1 General information 3 6.2 Handling. 3 6.3 Packaging 4 6.4 Toxins and other hazardous materials 7 Specimen considerations. .4 7.1 History of the specimen 4 7.2 Information sought. 4 7.3 Categories of specimen. 4 8 Sources of specimen contamination 55 8.1 Samplepreparation 8.2 Tools 5 8.3 Sample handling 5 8.3.1 General. 5 8.3.2 Exposureto gases. 5 8.3.3 Minimize contamination of the analysis area 6 8.4 Separation between neighbouring areas 6 9 Specimen storage and transfer of biomaterials 6 9.1 Storage 6 9.1.1 Storagetime 6 9.1.2 Descriptive list of containers for biomaterials 6 9.2 Temperatureand humidity 6 10 Education of specimen owner on appropriate specimen handlingprocedures .7 11 Specimenmountingproceduresofbiomaterials .7 12 Methods for reducing specimen charging .7 13 Specimenpreparationtechniquesofbiomaterials 7 Mechanical separation 7 13.1 13.2 Sectioning techniques. 7 13.3 Solvents forbiomaterials .7 13.4 Chemical etching 7 13.5 Ion sputtering. 7 14 Fracturing, cleaving and scribing. .7 15 Specimen-handlingtechniques .8 Bibliography .9 @ IS0 2021 - All rights reserved iii